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Item #:
220674748
Dulbecco's Phosphate Buffered Saline, 1X DPBS Buffer Without Calcium and Magnesium, pH 7.4, 0.1m Filtered Sterilization, 500ml/Bottle, 20 Bottle/Case
Item #:
220674748
KYD 172
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Product Details
| Item Weight | 1.1 lbs (500 grams) |
Product Description
Dulbecco's Phosphate Buffered Saline, 1X DPBS Buffer Without Calcium and Magnesium, pH 7.4, 0.1m Filtered Sterilization, 500ml/Bottle, 20 Bottle/Case
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Customer Questions & Answers
-
Question:
What is Dulbecco's Phosphate Buffered Saline (DPBS)?
Answer: Dulbecco's Phosphate Buffered Saline (DPBS) is a saline solution that maintains the pH and osmotic balance in biological research. This specific formulation is free from calcium and magnesium ions, making it suitable for various cell culture applications. It serves as a transport medium for cells and tissues, and is commonly used in laboratories for rinsing cells, diluting substances, and resuspending cells prior to further experiments. -
Question:
How do I use Dulbecco's Phosphate Buffered Saline in my laboratory?
Answer: To use DPBS, simply measure the required volume and pour it into your petri dish or cell culture flask. It can be utilized to wash cells, prepare samples, or maintain osmotic balance during experiments. Be sure to adhere to sterile techniques while handling, as contamination can affect experimental results. For example, you might use it to rinse adherent cell cultures before trypsinization or during cell count procedures. -
Question:
Can I store Dulbecco's Phosphate Buffered Saline, and how?
Answer: Yes, DPBS should be stored at room temperature, away from direct sunlight, and used within a certain timeframe after opening to maintain its sterility and efficacy. Proper storage helps preserve the quality of the buffer, ensuring reliable results in experiments. For best practices, consider sealing the bottle securely after use to prevent contamination, and label with the opening date to keep track of its usability. -
Question:
Is Dulbecco's Phosphate Buffered Saline suitable for all types of cells?
Answer: While DPBS is widely used for many cell types, its suitability may vary depending on specific cellular requirements. Its lack of calcium and magnesium makes it appropriate for certain applications like rinsing or transporting cells without causing activation or aggregation. Always check the compatibility with your specific cell line or application to confirm it's the right choice, especially for sensitive primary cells. -
Question:
What is the significance of the pH level in Dulbecco's Phosphate Buffered Saline?
Answer: The pH level of 7.4 in DPBS is crucial for maintaining physiological conditions that support cell viability and function. Deviations in pH can lead to cellular stress and affect experimental outcomes. By ensuring a stable pH, researchers can create an environment that mimics conditions in the human body, which is particularly important in cell culture and drug testing. -
Question:
What does '0.1 m Filtered Sterilization' mean in the product?
Answer: The term '0.1 m Filtered Sterilization' indicates that the DPBS solution has been filtered through a 0.1-micron filter, effectively removing any bacteria or particulate matter, thereby ensuring sterility. This process is essential for laboratory work where contamination could compromise results. Using pre-filtered solutions can save time and effort in preparing sterile media, facilitating cleaner experimental conditions. -
Question:
Can Dulbecco's Phosphate Buffered Saline be used for cell preservation?
Answer: While DPBS is primarily used for cell rinsing and transport, it is not ideal for long-term cell preservation. Instead, it can be used temporarily to maintain cells in culture before transferring them to preservation media like DMSO or glycerol. In practice, you might use DPBS to wash cells before cryopreservation, ensuring they are in optimized conditions for freezing. -
Question:
What applications are best suited for Dulbecco's Phosphate Buffered Saline?
Answer: DPBS is frequently used in a variety of applications such as cell culture, tissue processing, and in vitro diagnostic testing. Its properties make it ideal for washing cells, diluting biochemical assays, and maintaining osmotic balance. For instance, during immunostaining procedures, DPBS can help facilitate the washing steps without interfering with the binding of antibodies. -
Question:
Is Dulbecco's Phosphate Buffered Saline available without preservatives?
Answer: Yes, Dulbecco's Phosphate Buffered Saline is typically offered in a formulation that does not contain preservatives, ensuring that it does not affect cell growth or experimental results. When choosing a saline buffer for sensitive experiments, it’s critical to select products that are free from additives that could interfere with your assays or cell lines. Therefore, always double-check the product specifications to confirm its composition. -
Question:
Where can I buy Dulbecco's Phosphate Buffered Saline in Cayman Islands?
Answer: You can conveniently purchase Dulbecco's Phosphate Buffered Saline from Ubuy in Cayman Islands. Ubuy offers a wide range of laboratory supplies and ensures that you have easy access to high-quality products like DPBS. Their platform is user-friendly, and you can typically find various brands and formulations, giving you options suited to your specific laboratory needs.
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KYD 172
This product is not Fulfilled by Ubuy and can take minimum 10 days in delivery. We might cancel the product from the order and refund you if any issue arise with the delivery of this product.
This item is not restrict in my country.(Please click on above link if this item is not restrict in your country, So our team will review and allow.)
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Features & Benefits
- Advanced Technology: pH=7.4±0.1, sterilized for reliability.
- No Calcium & Magnesium: Prevents interference in sensitive experiments.
- Widespread Use: Ideal for cell dissociation and tissue sample transportation.
- Ready-to-Use: Eliminates preparation time for streamlined workflows.
- Long Shelf Life: 3 years at room temperature for ongoing research needs.
- Consistent Results: Ensures controlled ion conditions for reliable outcomes.
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